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Course Information
Course Unit Title : Electrophoresis Techniques
Course Unit Code : 01SAI5154
Type of Course Unit : Optional
Level of Course Unit : Second Cycle
Year of Study : Preb
Semester : 255.Semester
Number of ECTS Credits Allocated : 6,00
Name of Lecturer(s) :
Course Assistants :
Learning Outcomes of The Course Unit : 1) Definition and importance of electrophoresis
2) Electrophoresis application areas
3) Determination of the total mikroprotein content of muscle of fish
4) Defining with SDS PAGE of fish muscle protein
5) Defining with SDS PAGE of bacterial protein
Mode of Delivery : Face-To-Face
Prerequisities and Co-requisities Courses : Unavailable
Recommended Optional Programme Components : Unavailable
Course Contents : Prenciples of Electrophoresis, Electrophoresis variety, Agarose gel electrophoresis, Isoelectric focusing, SDS-Polyacrylamide gel electrophoresis, Two-dimensional electrophoresis
Languages of Instruction : Turkish
Course Goals : The ability of the laboratory of SDS PAGE to be able to apply
Course Aims : The aim of this course, electrophoresis types and specific electrophoretic methods to teach is intended to be practical.
WorkPlacement   Not Available
Recommended or Required Reading
Textbook : Bollang, D.M., Edelstein, S.J., 1993. ?Protein Methods? Wiley-Liss, Sons, INC., Publications. 229pp, New York.
Additional Resources : Andrews., A.T., 1988. ?Electrophoresis : Theory, techniques, Biochemical and Clinical Applications, Oxford University Press, Oxford.
Westermeier, R., 2005. Elecrtophoresis in Practice. Wiley-VCH Verlag GmbH Co. KGaA. 406p.
Material Sharing
Documents : Powerpoint presentations
Assignments : Electrophoretic analysis of studies made in seafood
Exams : Midterm and Final examination
Additional Material :
Planned Learning Activities and Teaching Methods
Lectures, Practical Courses, Presentation, Seminar, Project, Laboratory Applications (if necessary)
ECTS / Table Of Workload (Number of ECTS credits allocated)
Student workload surveys utilized to determine ECTS credits.
Activity :
Number Duration Total  
Course Duration (Excluding Exam Week) :
14 4 56  
Time Of Studying Out Of Class :
14 4 56  
Homeworks :
1 18 18  
Presentation :
0 0 0  
Project :
0 0 0  
Lab Study :
0 0 0  
Field Study :
0 0 0  
Visas :
1 20 20  
Finals :
1 20 20  
Workload Hour (30) :
30  
Total Work Charge / Hour :
170  
Course's ECTS Credit :
6      
Assessment Methods and Criteria
Studies During Halfterm :
Number Co-Effient
Visa :
1 70
Quiz :
0 0
Homework :
1 30
Attendance :
0 0
Application :
0 0
Lab :
0 0
Project :
0 0
Workshop :
0 0
Seminary :
0 0
Field study :
0 0
   
TOTAL :
100
The ratio of the term to success :
50
The ratio of final to success :
50
TOTAL :
100
Weekly Detailed Course Content
Week Topics  
1 Basic Prenciples and mean of Electrophoresis
  Study Materials: Andrews., A.T., 1988. ?Electrophoresis : Theory, techniques, Biochemical and Clinical Applications, Oxford University Press, Oxford.
Westermeier, R., 2005. Elecrtophoresis in Practice. Wiley-VCH Verlag GmbH Co. KGaA. 406p.
2 Use of electrophoresis in protein and amino acid analyses
  Study Materials: Andrews., A.T., 1988. ?Electrophoresis : Theory, techniques, Biochemical and Clinical Applications, Oxford University Press, Oxford.
3 Buffers
Salts, metal ions, and chelators
reducing agents
  Study Materials: Bollang, D.M., Edelstein, S.J., 1993. ?Protein Methods? Wiley-Liss, Sons, INC., Publications. 229pp, New York.
4 Protein sample preparation
Protein extraction
Homogenization
Sonication
French pressure cell
Enzymatic treatment
Other lysis methods
  Study Materials: Bollang, D.M., Edelstein, S.J., 1993. ?Protein Methods? Wiley-Liss, Sons, INC., Publications. 229pp, New York.
5 Protein Concentration determination
Bradford assay
Lowry assay
  Study Materials: Bollang, D.M., Edelstein, S.J., 1993. ?Protein Methods? Wiley-Liss, Sons, INC., Publications. 229pp, New York.
6 Electrophoresis variety
  Study Materials: Andrews., A.T., 1988. ?Electrophoresis : Theory, techniques, Biochemical and Clinical Applications, Oxford University Press, Oxford.
7 Agarose gel electrophoresis
  Study Materials: Andrews., A.T., 1988. ?Electrophoresis : Theory, techniques, Biochemical and Clinical Applications, Oxford University Press, Oxford.
8 Isoelectric focusing
  Study Materials: Andrews., A.T., 1988. ?Electrophoresis : Theory, techniques, Biochemical and Clinical Applications, Oxford University Press, Oxford.
9 Polyacrylamide gel electrophoresis of proteins (PAGE)
  Study Materials: Bollang, D.M., Edelstein, S.J., 1993. ?Protein Methods? Wiley-Liss, Sons, INC., Publications. 229pp, New York.
10 SDS-Polyacrylamide gel electrophoresis (SDS PAGE)
Equipment)
Preparing the gel
Pouring a gel
Preparing and loading samples
running the gel
  Study Materials: Bollang, D.M., Edelstein, S.J., 1993. ?Protein Methods? Wiley-Liss, Sons, INC., Publications. 229pp, New York.
11 Staining the gel with Coomassie Blue
Drying a gel
  Study Materials: Bollang, D.M., Edelstein, S.J., 1993. ?Protein Methods? Wiley-Liss, Sons, INC., Publications. 229pp, New York.
12 SDS PAGE
Determining protein molecular weight
  Study Materials: Bollang, D.M., Edelstein, S.J., 1993. ?Protein Methods? Wiley-Liss, Sons, INC., Publications. 229pp, New York.
13 Two-dimensional electrophoresis
  Study Materials: Andrews., A.T., 1988. ?Electrophoresis : Theory, techniques, Biochemical and Clinical Applications, Oxford University Press, Oxford.
Westermeier, R., 2005. Elecrtophoresis in Practice. Wiley-VCH Verlag GmbH Co. KGaA. 406p.
14 General evaluation